胰化大豆硫酸镁琼脂TSAM干粉培养基配置

发布时间:

2022-12-27

作者:

日水生物


胰化大豆硫酸镁琼脂TSAM Agar干粉培养基配制方法:称取本品41.5g于1L蒸馏水或去离子水中(可按比例增加或减少配制量),加热溶解,分装,121℃高压灭菌15分钟,灭菌结束后请摇匀,以防琼脂沉积于底部而凝固,倾注平板。
 
配方: g/L
 
胰蛋白胨:15.0g
大豆蛋白胨:5.0g
氯化钠:5.0g
硫酸镁:1.5g 
琼脂:15.0g 
pH7.3±0.2(25℃)
Soybean Magnesium Sulfate agar TSAM dry powder medium preparation method: 41.5g in 1L distilled water or deionized water (can be added or reduced proportions in proportion), heat dissolving, sub loading, 121 centigrade sterilization for 15 minutes, after sterilization, please shake well in order to prevent agar from the bottom and solidify, pour flat plate.
Formula: g/L
Tryptone: 15.0g
Soybean peptone: 5.0g
Sodium chloride: 5.0g
Magnesium Sulfate: 1.5g
Agar: 15.0g
PH7.3 + 0.2 (25 C)