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菌落总数的几个概念

作者:日水生物来源:培养基厂家 日期:2016-09-14 10:16

 菌落总数:Aerobic Plate Count,食品检样经过处理,在一定条件下培养后(如培基、温度、时间、pH、需氧性质等)所得1mL(g)检样中形成菌落的总数。只包括一群在平板技术琼脂上生长发育的嗜中温需氧或兼性厌氧菌的菌落总数。菌落总数不等于细菌总数。

菌落:Colony,单个微生物在适宜固体培养基表面或内部生长繁殖到一定程度;形成肉眼可见有 一定细菌群落。

CFU: Colony Forming Units,菌落形成单位。

菌落计数方法:选取菌落数在30~300cfu之间,无蔓延生长的平板计数。低于30的记录具体菌落数,大于300的可记录为多不可计,每个稀释度采用两个平行的均数。有较大片状菌落生长者不宜采用,若片状小于平板一半时且余部均匀分布,则以半个平板菌落数2倍。报告无明显界限链状菌落每条单链视为一个菌落。

凝集反应

颗粒性抗原(细菌、红细胞等)与相应抗体结合,在电解质参与下所形成的肉眼可见的凝集现象,称为凝集反应(Agglutination reaction)。其中的抗原称为凝集原,抗体称为凝集素。直接凝集反应

颗粒性抗原如细菌和细胞与相应抗体直接结合所出现的反应。

 

 


 

Total number of colonies: Aerobic Plate Count, processed food inspection samples, under certain conditions after training (such as prudential, temperature, time, pH, Aerobic properties, etc.) from 1 ml (g) the total number of colonies formed in the test samples. The total number of colonies of mesophilic aerobes or facultative anaerobes growing and developing on AGAR plate technology is included. The total number of colonies is not equal to the total number of bacteria.
 
Colony: Colony, where a single microorganism grows to a certain extent on or within a suitable solid medium; There is a certain community of bacteria visible to the naked eye.
 
CFU: Colony Forming Units, Colony Forming Units.
 
Colony counting method: the number of colonies was selected between 30 and 300cfu, and the number of plates with no spreading growth was selected. The number of specific colonies recorded below 30 is uncountable, and the number of colonies recorded above 300 is uncountable. Two parallel mean values are adopted for each dilution degree. It is not suitable for people with large pellets to grow. If the pellets are less than half of the plate and the rest are evenly distributed, the number of colonies of half plate is twice. There is no significant limit reported for each single chain of streptococcus colonies as a colony.
 
agglutination
 
Granular antigen (bacteria, red blood cells, etc.) binds to the corresponding antibodies, and the visible Agglutination phenomenon formed under the participation of electrolytes is called Agglutination reaction. The antigen is called agglutinin, and the antibody is called agglutinin. Direct agglutination
 
Reactions in which granular antigens such as bacteria and cells bind directly to the corresponding antibodies.

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该资讯的关键词为:菌落  培养基 

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