品红亚硫酸钠培养基配方

发布时间:

2022-12-27

作者:

山东培养基公司日水生物


品红亚硫酸钠培养基配制方法:称取本品5.45g于100ml蒸馏水或去离子水中,添加无水乙醇2ml,115℃高压灭菌20分钟,灭菌结束后请摇匀,以防琼脂沉积于器皿底部而凝固,备用。

配方成分说明:g/L
蛋白胨:10.0g,酵母浸粉:5.0g,牛肉浸粉:5.0g,乳糖:10.0g,磷酸氢二钾:3.5g,亚硫酸钠:5.0g,碱性品红:1.0g,琼脂:15.0g,pH7.3±0.1(25℃)。
 
品红亚硫酸钠培养基用法:称取本品5.45g于100ml蒸馏水或去离子水中(可按比例增加或减少配制量),添加无水乙醇2ml,115℃高压灭菌20分钟,灭菌结束后请摇匀,以防琼脂沉积于器皿底部而凝固,备用。
 
质控方法:理化检测,干粉(浅紫色);水分(≤6%);平板(橙红色);pH(7.3±0.1)。
生长检测:测生长率;观察菌落特征;35±2℃培养18-24小时。
菌种名称。接种量和生长特征:
生长特征,大肠埃希氏菌ATCC25922,30~100 cfu,生长率≥70%;菌落直径≥1.5mm;有金属光泽。
大肠埃希氏菌CMCC(B)44568,30~100 cfu,生长率≥70%;菌落直径≥1.5mm;有金属光泽。
产气肠杆菌ATCC13048,30~100 cfu,生长率≥70%;菌落直径≥1.5mm;红色菌落。
金黄色葡萄球菌ATCC25923,105~106cfu,抑制。
 
Fuchsin Basic Sodium Sulfite Agar Formula:g/L
Peptone:10.0g
Yeast Extract:5.0g
Beef Extract:5.0g
Lactose:10.0g
Dipotassium phosphate:3.5g
Sodium Sulfite:5.0g
Basic Fuchsin:1.0g
Agar:15.0g
pH7.3±0.1(25℃) 
Method of preparing fuchsin sodium sulfite medium: weigh 5.45g in 100 ml distilled water or deionized water, add 2 ml of absolute ethanol, sterilize under high pressure at 115 C for 20 minutes, shake well after sterilization, in order to prevent agar from depositing on the bottom of the vessel and solidifying, reserve.
Usage of Fuchsin Sodium Sulfite Medium: Weigh 5.45g in 100 ml distilled water or deionized water, add 2 ml of absolute ethanol, high-pressure sterilization at 115 C for 20 minutes, shake well after sterilization, in order to prevent agar from depositing on the bottom of the vessel and solidifying, reserve.