青岛日水生物logo图

0532-86107146

400 - 6177 -016

二维码

您现在的位置:首页 >> 技术服务 >> 培养基知识问答库 >> 白色念珠菌检查方法
技术服务

白色念珠菌检查方法

作者:日水生物来源:培养基厂家 日期:2016-09-14 10:11

 菌株培养:

1、增菌培养   取供试液10ml(相当于供试品1g、ml、10cm2)直接或处理后接种至适量(不少于100ml)的沙氏葡萄糖肉汤培养基中,培养48~72小时。

2、分离培养    取上述培养物划线接种于沙氏葡萄糖琼脂培养基平板上,培养24~48小时(必要时延长至72小时)。

结果判断:

1、白色念珠菌在沙氏葡萄糖琼脂培养基上生长的菌落呈乳白色偶见淡黄色,表面光滑有浓酵母气味,培养时间稍久则菌落增大,颜色变深、质地变硬或有皱摺。若平板上无菌落生长或生长的菌落与上述菌落形态特征不符,判供试品未检出白色念珠菌。

2、如平板上生长的菌落与上述菌落形态特征相符或疑似,应挑选2~3个菌落分别接种至念珠菌显色培养基平板上,培养24~48小时(必要时延长至72小时)。若平板上无绿色或翠绿色的菌落生长,判供试品未检出白色念珠菌。

3、若平板上生长的菌落为绿色或翠绿色,挑取相符或疑似的菌落接种于1%吐温80玉米琼脂培养基上,培养24~48小时。取培养物进行染色,镜检及芽管试验。

4、芽管试验 挑取1%吐温80玉米琼脂培养基上的培养物,接种于加有一滴血清的载玻片上,盖上盖玻片,置湿润的平皿内,置35~37℃、1~3h,置显微镜下观察,可见到由孢子长出短小芽管。

5、若上述疑似菌为非革兰阳性菌,显微镜未见厚膜孢子、假菌丝、芽管,判供试品未检出白色念珠菌。

 


 

Strain culture:
 
1, take the test liquid enrichment culture 10 ml (the equivalent of 1 g, ml, of the 10 cm2) directly or after inoculation to moderate (not less than 100 ml) of savary-gilliard glucose broth medium, cultivate 48 ~ 72 hours.
 
2. The above cultures were separated and inoculated on the sa glucose AGAR medium plate, and cultured for 24-48 hours (extended to 72 hours if necessary).
 
Result judgment:
 
1, candida albicans in savary-gilliard glucose AGAR culture medium on the growth of colony has milk-white accidentally see pale yellow, smooth surface has a strong smell, yeast cultivation time slightly long increases colony, darker color, texture, harden or buckling. If there is no colony growth or growth on the plate and the above colony morphological characteristics are inconsistent, it is judged that no candida albicans has been detected in the test product.
 
2, such as tablet on the growth of colonies are in conformity with the colony morphology or suspected, should choose 2 ~ 3 colonies were inoculated to candida color medium plate, cultivate 24 to 48 hours (ShiYanChang necessary to 72 hours). If there is no green or greenish-green colony growth on the plate, no candida albicans was detected in the test product.
 
3. If the bacterial colonies on the plate are green or emerald green, select suitable or suspected colonies and inoculate them on 1% tween 80 corn AGAR medium for 24-48 hours. Culture materials were used for dyeing, microscopic examination and bud tube test.
 
4, 80 corn germ tube test pick 1% twain AGAR culture medium, inoculated on the slide with a drop of blood serum added, cover glass, in wet plate, buy 35 ~ 37 ℃, 1 ~ 3 h, microscope, visible to grow short and germ tube by spores.
 
5. If the above suspected bacteria are non-gram positive bacteria, no thick film spores, pseudomycelia, and germ tubes have been observed under the microscope, and no candida albicans have been detected in the test products.

所属类别: 培养基知识问答库

该资讯的关键词为:白色念珠菌检查方法  日水培养基  微生物培养基 

  鲁ICP备16034020号  公司地址:山东省青岛市黄岛区黄河西路86号  电话:86-0532-86767039 86107146  Sitemap