胰酪大豆胨琼脂培养基TSA成分表

发布时间:

2022-12-27

作者:

青岛日水


药典 tsa配方 ,胰酪大豆胨琼脂培养基TSA干粉配制方法:称取本品40.0g于1L蒸馏水或去离子水中[可按比例增加或减少配制量],加热搅拌煮沸至完全溶解,分装121℃高压灭菌15分钟,灭菌结束后请摇匀,以防琼脂沉积于器皿底部而凝固,备用。

 
Tryptic Soy Peptone Agar Medium Formula[配方]: g/L
Pancreatic Digest of  Casein [胰酪胨]:15.0g                         
Sodium Chloride[氯化钠]: 5.0g               
Enzymatic Digest of Soybean Meal[大豆木瓜蛋白酶水解物]:5.0g                                                   
Agar[琼脂]:15.0g                       
pH7.3±0.2[25℃]
 
The preparation method of TSA dry powder of pancreatic cheese and soybean Peptone Agar Culture medium: weigh 40.0g in 1L distilled water or deionized water [can increase or reduce the amount of preparation in proportion], heat and stir to boil until completely dissolved, separate into 121 C high pressure sterilization for 15 minutes, shake well after sterilization, so as to prevent agar from depositing in the bottom of the vessel and solidifying, reserve.
Tryptic Soy Peptone Agar Medium Formula[formula]:g/L
Pancreatic Digest of Casein [tryptone]:15.0g]
Sodium Chloride[NaCl]:5.0g
Enzymatic Digest of Soybean Meal[]:5.0g hydrolysate of soybean papain]:5.0g
Agar[agar]:15.0g
PH7.3 + 0.2[25 C]