TTC卵磷脂吐温80营养琼脂培养基基础配方

发布时间:

2022-12-27

作者:

青岛日水生物


TTC卵磷脂吐温80营养琼脂培养基基础配制方法:称取本品44.0g于1L蒸馏水或去离子水中,加入1支20107[若配制TTC卵磷脂-吐温80营养琼脂培养基,每100ml基础中还需添加1支20101],加热煮沸至完全溶解,分装,121℃高压灭菌20分钟,灭菌结束后请摇匀,以防琼脂沉积于器皿底部而凝固,备用。

 
Lecithin Tween-80 Nutrient Agar Base Formula[配方]: g/L
Peptone[蛋白胨]:20.0g
Beef Extract[牛肉浸粉]:3.0g
Lecithin[卵磷脂]:1.0g
Sodium Chloride[氯化钠]:5.0g
Agar[琼脂]:15.0g
pH7.3±0.1[25℃]
 
TTC lecithin Tween 80 nutrient agar medium preparation method: weigh 44.0g in 1L distilled water or deionized water, add a 20107 [if the preparation of TTC lecithin Tween 80 Nutrient Agar medium, every 100ml of the base also need to add a 20101], heat boiling to complete dissolution, sub-packaging, high pressure sterilization at 121 for 20 minutes, sterilization After the fungus is finished, shake well to prevent the agar from depositing on the bottom of the utensils and solidify.
Lecithin Tween-80 Nutrient Agar Base Formula[formula]:g/L
Peptone[peptone]:20.0g
Beef Extract[beef dip powder]:3.0g
Lecithin[lecithin]:1.0g
Sodium Chloride[NaCl]:5.0g
Agar[agar]:15.0g
PH7.3 + 0.1[25 C]