Tergitol-7琼脂干粉的配方

发布时间:

2022-12-27

作者:


Tergitol-7琼脂培养基配制方法:称取本品56.2g于1L蒸馏水或去离子水中(可按比例增加或减少配制量),加热煮沸至完全溶解,分装,121℃高压灭菌15分钟,冷却至50℃左右时,每100mL培养基中加入1支20101(0.5%TTC溶液,1mL),混匀倒入无菌平皿,备用。 资料来自青岛日水生物。
 
配方: g/L
 
胰酪蛋白胨:10.0g
酵母浸粉:6.0g
牛肉浸粉:5.0g
乳糖:20.0g
溴麝香草酚蓝:0.05g
Tergitol-7:0.1g
琼脂:15.0g
pH7.0±0.2(25℃)
Tergitol-7 agar medium preparation method: This product is called 56.2g in 1L distilled water or deionized water (can increase or reduce the dosage in proportion), heat and boil to completely dissolve, pack, sterilize at 121 centigrade for 15 minutes, cooling to about 50 C, adding 1 branches and 20101 (0.5%TTC solution, 1mL) in every 100mL medium, and mixing into asepsis Plate, spare.
Formula:g/L
Casein peptone: 10.0g
Yeast extract: 6.0g
Beef dipping: 5.0g
Lactose: 20.0g
Bromothymol blue: 0.05g
Tergitol-7:0.1g
Agar: 15.0g
PH7.0 + 0.2 (25 C)