配置Endo培养基干粉粉剂方法

发布时间:

2022-12-27

作者:

青岛日水生物


Endo培养基配制方法:称取本品3.86g于98ml蒸馏水或去离子水中并加入2ml无水乙醇(可按比例增加或减少配制量),121℃高压灭菌15分钟,摇匀,备用。
 
配方): g/L
 
蛋白胨:10.0g
乳糖:10.0g
磷酸氢二钾:2.5g
亚硫酸钠:3.3g
碱性品红:0.3g
琼脂:12.5g
pH7.4±0.2(25℃)
Endo culture medium preparation method: take this product 3.86g in 98ml distilled water or deionized water and add 2ml anhydrous ethanol (can increase or reduce the amount of preparation according to proportion), 121 centigrade high pressure sterilization for 15 minutes, shake up and spare.
Formula):g/L
Peptone: 10.0g
Lactose: 10.0g
Two potassium hydrogen phosphate: 2.5G
Sodium sulfite: 3.3g
Basic fuchsin: 0.3g
Agar: 12.5g
PH7.4 + 0.2 (25 C)