氯化镁孔雀绿增菌液干粉的制备说明

发布时间:

2022-12-27

作者:

日水生物


氯化镁孔雀绿增菌液[MM]干粉培养基配置方法:称取本品31.5g于1L蒸馏水或去离子水中,加热煮沸至完全溶解,分装,115℃高压灭菌15分钟,备用。
 
Formula[配方]: g/L
 
Enzymatic Digest of Soybean  Meal:5.0g
[大豆蛋白胨] 
Sodium Chloride[氯化钠]:8.0g
Monopotassium phosphate:1.4g
[磷酸二氢钾] 
Dipotassium phosphate:0.2g
[磷酸氢二钾] 
Anhydrous magnesium chloride:16.86g
[无水氯化镁] 
Malachite green[孔雀绿]: 0.036g         
pH5.1±0.2 [25℃]
The configuration method of [MM] dry powder medium of magnesium chloride malachite green bacteria: called 31.5g in 1L distilled water or deionized water, heated and boiled to completely dissolving, packing, sterilizing at 115 C for 15 minutes.
Formula[formula]:g/L
Enzymatic Digest of Soybean Meal:5.0g
[soybean peptone]
Sodium Chloride[sodium chloride]: 8.0g
Monopotassium phosphate:1.4g
[potassium dihydrogen phosphate]
Dipotassium phosphate:0.2g
[two potassium hydrogen phosphate]
Anhydrous magnesium chloride:16.86g
[anhydrous magnesium chloride]
Malachite green[malachite green]: 0.036g
PH5.1 + 0.2 [25 C]