WS琼脂干粉培養基的制备方法

发布时间:

2022-12-27

作者:

日水生物


WS琼脂粉剂培養基配制方法:称取本品65.76g于1L蒸馏水或去离子水中,搅拌煮沸1分钟使完全溶解,冷却至50—55℃倾注平板。
 
配方): g/L
 
月示蛋白胨:12.0g
牛肉浸出粉:3.0g
氯化钠:5.0g
乳糖:12.0g
蔗糖:12.0g
十二烷基硫酸钠:2.0g
酸性复红:0.1g
溴麝香草酚蓝:0.064g
硫代硫酸钠:6.8g
柠檬酸铁铵:0.8g
琼脂:12.0g
pH7.5±0.1(℃)
WS agar powder medium preparation method: This product is called 65.76g in 1L distilled water or deionized water, stirring and boiling for 1 minutes to completely dissolve, cooling to 50 - 55 degrees centigrade to pour plate.
Formula):g/L
Monthly peptone: 12.0g
Beef extract powder: 3.0g
Sodium chloride: 5.0g
Lactose: 12.0g
Sucrose: 12.0g
Twelve sodium alkyl sulfate: 2.0g
Acid reddish: 0.1g
Bromothymol blue: 0.064g
Sodium thiosulfate: 6.8g
Ferric citrate: 0.8g
Agar: 12.0g
PH7.5 + 0.1 (c)