亚硫酸铋琼脂培养基配方

发布时间:

2022-12-27

作者:

日水生物


亚硫酸铋琼脂培养基的配制方法:称取本品5.23g于100ml蒸馏水或去离子水中,搅拌煮沸溶解1分钟,定容,冷却至50—55℃倾注平板。建议每次配制不超过200ml,配制后隔夜使用且在3天内用完。
 
配方): g/L
 
胰酪蛋白胨:5.0g
牛肉浸粉:5.0g
动物组织消化物:5.0g
葡萄糖:5.0g
硫酸亚铁:0.3g
磷酸氢二钠:4.0g
亚硫酸铋指示剂:8.0g
煌绿:0.025g
琼脂:20.0g
pH 7.6±0.2(25℃)
Preparation method of bismuth bismuth agar medium: This product is called 5.23g in 100ml distilled water or deionized water, stirring and boiling to dissolve for 1 minutes, fixed volume, and cooling to 50 - 55 degrees centigrade. It is suggested that each formulation should not exceed 200ml, and be used overnight after preparation and run out in 3 days.
Formula):g/L
Casein peptone: 5.0g
Beef dipping: 5.0g
Animal tissue digestion: 5.0g
Glucose: 5.0g
Ferrous sulfate: 0.3g
Two sodium hydrogen phosphate: 4.0g
Bismuth bismuth indicator: 8.0g
Brilliant green: 0.025g
Agar: 20.0g
PH 7.6 + 0.2 (25 C)