麦康凯琼脂的配方是什么？
发布时间：
2022-12-27
作者：
日水生物
麦康凯琼脂配制方法:称取本品50.0g于1L蒸馏水或去离子水中，加热煮沸至完全溶解，分装，121℃高压灭菌15分钟,灭菌结束后请摇匀，以防琼脂沉积于器皿底部而凝固，备用。资料来自日水生物
Formula(麦康凯琼脂配方)： g/L
Pancreatic Digest of Casein\胰酪蛋白胨：1.5g
Pancreatic Digest of Gelatin\明胶蛋白胨：17.0g
Peptic Digest of Animal Tissue\动物组织消化物：1.5g
Lactose\乳糖：10.0g
Bile Salt\牛胆盐：1.5g
Sodium Chloride\氯化钠：5.0g
Crystal Violet\结晶紫：0.001g
Neutral Red\中性红：0.03g
Agar\琼脂：13.5g
pH7.1±0.2(25℃)
Mae Kang Kai agar preparation method: call this product 50.0g in 1L distilled water or deionized water, heat and boil to completely dissolve, pack, 121 centigrade high pressure sterilization for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the vessel and solidified, spare. Data from diurnal aquatic organisms
Formula (formula):g/L
Casein peptone: 1.5g
Gelatin peptone: 17.0g
Animal tissue digestion: 1.5g
Lactose: 10.0g
Bovine bile salt: 1.5g
Sodium chloride: 5.0g
Crystal violet: 0.001g
Neutral red: 0.03g
Agar: 13.5g
PH7.1 + 0.2 (25 C)