Muller -Kauffmann四硫磺酸钠肉汤基础成分说明

发布时间:

2022-12-27

作者:

日水生物


Muller -Kauffmann 四硫磺酸钠肉汤基础配制方法:称取本品105.8g于1L蒸馏水或去离子水中,使用盐酸调节pH至7.0±0.2(25℃)加热煮沸1分钟,冷至25℃,每100ml中加入20315、20316各一支,混匀,分装。
 
Formula(配方): g/L
 
Peptone\蛋白胨:10.0g
Beef Extract\牛肉浸粉:5.0g
Sodium Chloride\氯化钠:3.0g
Oxgall\牛胆汁:4.7g
Calcium Carbonate\碳酸钙:45.0g
Sodium Thiosulfate\硫代硫酸钠:38.1g
Muller -Kauffmann four sodium sulfonic acid sodium broth preparation method: called the product 105.8g in 1L distilled water or deionized water, using hydrochloric acid to adjust pH to 7 + 0.2 (25) heating boil for 1 minutes, cold to 25 degrees, each 100ml added 20315, 20316, mix, separate.
Formula (formula):g/L
Peptone: 10.0g
Beef dipping: 5.0g
Sodium chloride: 3.0g
Bovine bile: 4.7G
Calcium carbonate: 45.0g
Sodium thiosulfate: 38.1g