LB琼脂的配方 lb agar,Miller配制

发布时间:

2022-12-27

作者:

日水生物


LB琼脂干粉培养基的配制方法: 称取本品40.0g于1L蒸馏水或去离子水中,加热溶解,分装,121℃高压灭菌15分钟,灭菌后请迅速摇匀,以防琼脂沉于器皿底部而凝固,备用。
 
Formula(配方): g/L
 
Tryptone/胰蛋白胨:10.0g
Yeast Extract/酵母浸粉:5.0g
Sodium Chloride/氯化钠:10.0g
Agar/琼脂:15.0g
pH7.0±0.2(25℃)
The preparation method of LB agar dry powder medium: call this product 40.0g in 1L distilled water or deionized water, heat and dissolve, pack, sterilize at 121 centigrade for 15 minutes, and shake well after sterilization, so as to prevent agar from sinking at the bottom of the utensils and solidify. Spare.
Formula (formula):g/L
Tryptone: 10.0g
Yeast extract: 5.0g
Sodium chloride: 10.0g
Agar: 15.0g
PH7.0 + 0.2 (25 C)