2×YT培养基的配方是什么?

发布时间:

2022-12-27

作者:

日水生物


2×YT培养基的配方配制方法: 称取本品31.0g于1L蒸馏水或去离子水中,加热煮沸至完全溶解,分装,121℃高压灭菌15分钟,备用。
 
Formula(配方): g/L
Pancreatic Digest of  Casein/胰酪蛋白胨:16.0g
Yeast Extract/酵母浸粉: 10.0g
Sodium Chloride/氯化钠:5.0g
pH7.0±0.2 (25℃)
2 x YT medium formula preparation method: the product is called 31.0g in 1L distilled water or deionized water, heated and boiled to complete dissolving, packing, sterilizing at 121 centigrade for 15 minutes.
Formula: g/L
Casein peptone: 16.0g
Yeast extract: 10.0g
Sodium chloride: 5.0g
PH7.0 + 0.2 (25 C)