溴甲酚紫乳糖琼脂干粉培养基的配制
发布时间：
2022-12-27
作者：
日水生物
溴甲酚紫乳糖琼脂干粉培养基配制方法: 称取本品31.0g于1L蒸馏水或去离子水中，加热煮沸至完全溶解，分装,121℃高压灭菌15分钟,灭菌结束后请摇匀，以防琼脂沉积于器皿底部而凝固，备用。
Formula/配方： g/L
Tryptone/胰蛋白胨:5.0g
Lactose/乳糖:10.0g
Beef Extract/牛肉浸粉:3.0g
Bromcresol Purple/溴甲酚紫:0.025g
Agar /琼脂:13.0g
pH7.0±0.2 /25℃
Preparation method: call this product 31.0g in 1L distilled water or deionized water, heat and boil to completely dissolve, pack, sterilize at 121 centigrade for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils.
Formula: g/L
Tryptone: 5.0g
Lactose: 10.0g
Beef dipping: 3.0g
Bromo cresol violet: 0.025g
Agar: 13.0g
PH7.0 + 0.2 /25 C