DMEM培养基的成份有哪些？
发布时间：
2022-12-24
作者：
品牌/洁净室微生物培养基
DMEM是 dulbecco's modified eagle medium的缩写,是一种含各种氨基酸和葡萄糖的培养基，是在MEM培养基的基础上研制的。与MEM比较增加了各种成分用量，同时又分为高糖型（低于4500mg/L）和低糖型(低于1000mg/L)。高糖型有利于细胞停泊于一个位置生长，适于生长较快、附着较困难肿瘤细胞等。所以其特点主要包括以下：
（1）氨基酸含量为依格尔培养基的2倍,且含有非必需氨基酸,如甘氨酸等；
（2）维生素含量为依格尔培养基的4倍；
（3）含有糖酵解途径中的重要物质——丙酮酸；
（4）含有微量的铁离子.Eagle来自该培养基的发明人Harry Eagle
1640和DMEM培养基的区别
在组分和用途上有区别，主要是组分上的区别，查各自的配方表可知。用途上这两种应用都很广泛，且都适合很多种细胞的培养。
RPMI 1640主要用于悬浮细胞培养。可适用于哺乳动物、特殊造血细胞、正常或恶性增生的白细胞，杂交瘤细胞的培养。其它像K-562、HL-60、IM-9等成淋巴细胞、T细胞淋巴瘤细胞以及HCT-15上皮细胞等均可参考使用。
DMEM适用于贴壁细胞生长，如各种实体瘤贴壁细胞。
DMEM培养基的成份：
含量mg/L
无水氯化钙，200
硝酸铁9H20，0.1
氯化钾，400
无水硫酸二氢钠，125
L盐酸精氨酸，84
L盐酸胱氨酸，63
L谷氨酸胺，584
L甘氨酸，30
L盐酸组氨酸，42
L异亮氨酸，105
L亮氨酸，105
L甲硫氨酸，30
L苯丙氨酸，66
L丝氨酸，42
L苏氨酸，95
L色氨酸，16
L酪氨酸钠盐，104
L泛酸钙，4
氯化胆碱，4
叶酸，4
肌醇，7.2
核黄素，0.4
盐酸硫胺，4
盐酸吡哆辛，4
葡萄糖，4500
酚红，15
The composition of DMEM medium
DMEM is the abbreviation of dulbecco's modified Eagle medium. It is a medium containing various amino acids and glucose. It is developed on the basis of MEM medium. Compared with MEM, the amount of various ingredients was increased, and it was also divided into high glucose (less than 4500mg/L) and low sugar type (less than 1000mg/L). High glucose is beneficial for cells to grow at a single location, suitable for rapid growth, difficult adhesion and tumor cells. So its characteristics include the following:
(1) the amino acid content is 2 times higher than the agar medium and contains non essential amino acids, such as glycine.
(2) vitamin content was 4 times higher than the agar medium.
(3) pyruvic acid, an important substance in the glycolysis pathway.
(4) trace iron ions.Eagle came from the inventor Harry Eagle of the medium.
The difference between 1640 and DMEM medium
1640 there is a difference between the medium and the DMEM medium in terms of components and uses, mainly on the difference between components. These two kinds of media are widely used and are suitable for the cultivation of many kinds of cells.
RPMI 1640 is mainly used for suspension cell culture. It can be used for the culture of hybridoma cells in mammals, hematopoietic cells, normal or malignant hyperplasia. Other cells such as K-562, HL-60, IM-9 and other lymphoblastic cells, T cell lymphoma cells and HCT-15 epithelial cells can be used for reference.
DMEM is suitable for adherent cell growth, such as various solid tumor adherent cells.
The components of the DMEM medium:
Content mg/L
Anhydrous calcium chloride, 200
Iron nitrate 9H20, 0.1
Potassium chloride, 400
Anhydrous sulphuric acid, two sodium hydrogen, 125
L arginine hydrochloride, 84
L cystine hydrochloride, 63
L glutamic acid amine, 584
L glycine, 30
L HCl histidine, 42
L isoleucine, 105
L leucine, 105
L methionine, 30
L phenylalanine, 66
L serine, 42
L threonine, 95
L tryptophan, 16
L tyrosine sodium salt, 104
L calcium pantothenate, 4
Choline chloride, 4
Folic acid, 4
Inositol, 7.2
Riboflavin, 0.4
Thiamine hydrochloride, 4
Pyridoxine hydrochloride, 4
（pyridoxine hydrochloride在培养基中起什么作用）吡哆醇盐酸盐：本品是机体内很多酶如转氨基酶、脱羧酶、消旋酶、脱氢酶、合成酶和羟化酶等的辅酶，且可帮助糖类和蛋白质的分解、利用。缺乏时有类似烟酸的缺乏症。此外维生素B6还参与色氨酸将烟酸转化为5-羟色胺的反应。并可刺激白细胞的生长，是形成血红蛋白所需要的物质。
Glucose, 4500
Phenol red, 15