麦康凯琼脂的配方是什么?

发布时间:

2022-12-27

作者:

日水生物


麦康凯琼脂的配制方法:
称取本品57.0g于1L蒸馏水或去离子水中可按比例增加或减少配制量),加热煮沸至完全溶解,分装,121℃高压灭菌15分钟,灭菌结束后请摇匀,以防琼脂沉积于器皿底部而凝固,备用。
 
Formula配方: g/L
Peptone蛋白胨:20.0g
Lactose乳糖:10.0g
Bile Salts胆盐:5.0g
Sodium Chloride氯化钠:5.0g
Neutral Red中性红:0.025g
Crystal Violet结晶紫:0.001g
Agar琼脂:17.0g
pH7.2±0.2(25℃)
Preparation method:
Call this product 57.0g in 1L distilled water or deionized water to increase or reduce the amount of preparation), heat and boil to completely dissolve, pack, 121 degrees centigrade sterilization for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils and solidified. Spare.
Formula formula: g/L
Peptone peptone: 20.0g
Lactose lactose: 10.0g
Bile Salts bile salt: 5.0g
Sodium Chloride sodium chloride: 5.0g
Neutral Red neutral red: 0.025g
Crystal Violet crystal violet: 0.001g
Agar agar: 17.0g
PH7.2 + 0.2 (25 C)