胆汁七叶苷琼脂的配制方法是什么?

发布时间:

2022-12-27

作者:

日水生物


胆汁七叶苷琼脂干粉培养基配制方法:
称取本品44.5g于1L蒸馏水或去离子水中,加热煮沸至完全溶解,分装,121℃高压灭菌15分钟,灭菌结束后请摇匀,以防琼脂沉积于器皿底部而凝固,备用。
 
Formula胆汁七叶苷琼脂配方: g/L 
Peptone蛋白胨:8.0g
Bile Salts胆盐:20.0g
Ferric Citrate柠檬酸铁:0.5g
Aesculin七叶苷: 1.0g
Agar(琼脂:15.0g
pH7.1±0.2(25℃)
Preparation method:
Call this product 44.5g in 1L distilled water or deionized water, heat and boil to completely dissolve, pack, sterilize at 121 centigrade for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils.
Formula formula: g/L
Peptone peptone: 8.0g
Bile Salts bile salt: 20.0g
Ferric Citrate iron citrate: 0.5g
Aesculin seven leaf glucoside: 1.0g
Agar (agar: 15.0g)
PH7.1 + 0.2 (25 C)