DNA酶试验琼脂配制
发布时间：
2022-12-27
作者：
日水生物
DNA酶试验琼脂配制方法:
称取本品42.0g缓缓加热溶解于1L蒸馏水或去离子水中（避免形成不溶性丝状物），116℃高压灭菌15分钟,灭菌结束后请摇匀，以防琼脂沉积于器皿底部而凝固，冷却至50℃左右时，倾入无菌平皿中。
Formula(DNA酶试验琼脂配方)： g/L
Tryptone（胰蛋白胨：15.0g
Enzymatic Digest of Soybean Meal
（大豆胨：5.0g
Sodium Chloride（氯化钠：5.0g
Calcium Chloride （氯化钙：0.02g
DNA ： 2.0g
Agar（琼脂：15.0g
pH7.3±0.2（25℃）
Preparation method:
42.0g slowly heating dissolved in 1L distilled water or deionized water (to avoid the formation of insoluble filaments), 116 C sterilization for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the vessel and solidified, cooling to about 50 C, into a sterile flat dish.
Formula (formula):g/L
Tryptone (tryptone: 15.0g)
Enzymatic Digest of Soybean Meal
(soybean peptone: 5.0g
Sodium Chloride (sodium chloride: 5.0g)
Calcium Chloride (calcium chloride: 0.02g)
DNA:2.0g
Agar (agar: 15.0g)
PH7.3 + 0.2 (25 C)