Mueller-Hinton 琼脂配制方法

发布时间:

2022-12-27

作者:

日水生物


Mueller-Hinton 琼脂配置方法:称取本品38.0g于1L蒸馏水或去离子水中,煮沸溶解,分装,121℃高压灭菌15分钟,灭菌结束后请摇匀,以防琼脂沉积于器皿底部而凝固;若制备MH血琼脂,冷至50℃左右,无菌操作每100ml培养基中加入5ml脱纤维羊血,轻轻摇匀,倾注平板。  

 
FormulaMueller-Hinton 琼脂配置方法配方: g/L
 
Peptic Digest of Animal Tissue
动物组织酶解物 6.0g
Casein Hydrolysate
酪蛋白酶解物  17.5g
Soluble Starch可溶性淀粉 1.5g
Agar琼脂  13.0g
pH7.2±0.225℃)
 
38.0g in 1L distilled water or deionized water, boiling and dissolving, packing, sterilizing at 121 centigrade for 15 minutes. After sterilizing, please shake it evenly to prevent agar from depositing at the bottom of the utensils; if MH blood agar is prepared, cold to about 50 C, the aseptic operation is added into the 5ml defibrous sheep blood in every 100ml medium, gently shaking and pouring the flat plate.
Formula formula):g/L
Peptic Digest of Animal Tissue
Animal tissue enzyme hydrolysate 6.0g
Casein Hydrolysate
Casein hydrolysate 17.5g
Soluble Starch soluble starch 1.5g
Agar agar 13.0g
PH7.2 + 0.225 C)