庆大霉素琼脂基础培养基的配制

发布时间:

2022-12-27

作者:

日水生物


庆大霉素琼脂基础培养基的配制方法:
称取本品56.0g于1L蒸馏水或去离子水中(可按比例增加或减少配制量),121℃高压灭菌15分钟,冷却至50—55℃,每100ml培养基中添加1支20543,混匀,倾注平板。
 
Formula(庆大霉素琼脂基础培养基配方): g/L
 
Peptone(蛋白胨)                           10.0g
Beef Extract(牛肉浸出粉)             3.0g
Sodium Sulfite(亚硫酸钠)              3.0g
Sodium Citrate (柠檬酸钠)          10.0g
Sodium Chloride(氯化钠)                 5.0g
Sucrose(蔗糖)                                   10.0g     
Agar(琼脂)                                     15.0g
pH 8.4±0.1(25℃)
 
 

 
Preparation method:
According to take this product 56.0 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), 121 ℃ high pressure sterilization for 15 minutes, cool to 50 to 55 ℃, each 100 ml, 1-20543 is added to the medium blender, pour into the tablet.
 
Formula: g/L
 
Peptone (Peptone) 10.0g
Beef Extract 3.0g
Sodium Sulfite 3.0g
Sodium Citrate (Sodium Citrate) is 10.0g
Sodium Chloride (nacl) 5.0 g
Sucrose (Sucrose) 10.0g
Agar (Agar) 15.0g
PH 8.4 + / - 0.1 (25 ℃)