庆大霉素琼脂基础培养基的配制
发布时间:
2022-12-27
作者:
日水生物
庆大霉素琼脂基础培养基的配制方法:
称取本品56.0g于1L蒸馏水或去离子水中(可按比例增加或减少配制量),121℃高压灭菌15分钟,冷却至50—55℃,每100ml培养基中添加1支20543,混匀,倾注平板。
Formula(庆大霉素琼脂基础培养基配方): g/L
Peptone(蛋白胨) 10.0g
Beef Extract(牛肉浸出粉) 3.0g
Sodium Sulfite(亚硫酸钠) 3.0g
Sodium Citrate (柠檬酸钠) 10.0g
Sodium Chloride(氯化钠) 5.0g
Sucrose(蔗糖) 10.0g
Agar(琼脂) 15.0g
pH 8.4±0.1(25℃)
Preparation method:
According to take this product 56.0 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), 121 ℃ high pressure sterilization for 15 minutes, cool to 50 to 55 ℃, each 100 ml, 1-20543 is added to the medium blender, pour into the tablet.
Formula: g/L
Peptone (Peptone) 10.0g
Beef Extract 3.0g
Sodium Sulfite 3.0g
Sodium Citrate (Sodium Citrate) is 10.0g
Sodium Chloride (nacl) 5.0 g
Sucrose (Sucrose) 10.0g
Agar (Agar) 15.0g
PH 8.4 + / - 0.1 (25 ℃)