42℃ 生长试验用培养基配置配制

发布时间:

2022-12-27

作者:

日水生物


42℃ 生长试验用培养基配置配制方法:
称取本品55.0g于1L蒸馏水或去离子水中(可按比例增加或减少配制量),加热溶解,分装,121℃高压灭菌15分钟,备用。
 
Formula(42℃ 生长试验用培养基配方): g/L
 
Tryptone(胰胨)                                   17.0g
Enzymatic Digest of Soybean Meal
(大豆胨)                                                 3.0g
Sodium Chloride(氯化钠)                      30.0g 
Dipotassium Phosphate 
(磷酸氢二钾)                                             2.5g
Dextrose(葡萄糖)                                     2.5g
pH7.3±0.2(25℃)       
 
 

Preparation method:
According to take this product 55.0 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heat to dissolve, repackaging, 121 ℃ high pressure sterilization for 15 minutes, and set aside.
 
Formula: g/L
 
Tryptone (Tryptone) 17.0g
The Enzymatic Digest of Soybean Meal
(soya bean peptone) 3.0g
Sodium Chloride (nacl) 30.0 g
Dipotassium Phosphate
(dipotassium hydrogen phosphate) 2.5g
Dextrose 2.5g
PH7.3 + / - 0.2 (25 ℃)