CIN-1 培养基基础

发布时间:

2022-12-27

作者:

日水生物


CIN-1 培养基基础
 
配制方法:
    称取本品57.0g于1L蒸馏水或去离子水
中(可按比例增加或减少配制量),加热煮
沸至完全溶解,分装,121℃高压灭菌15分钟,冷却至50-55℃,每100ml培养基中添加1支20601(CIN-1培养基基础添加剂),混匀,倾注平板。
 
Formula(配方): g/L
Tryptone(胰胨)                                        20.0g                          
Sodium Chloride(氯化钠)                              1.0g
Yeast Extract(酵母浸粉)                          2.0g
Mannitol(甘露醇)                                    20.0g
Sodium Desoxycholate(去氧胆酸钠)     2.0 g
Magnesium Sulfate•7H2O 
(硫酸镁•7H2O )                                            0.01g
Irgasan(Irgasan)                                      0.0004g
Neutral Red(中性红)                                   0.03g
Crystal Violet(结晶紫)                              0.001g
Agar(琼脂)                                              12.0g                              
pH7.5±0.1(25℃)       
 
 
 

 
Preparation method:
Weigh 57.0g of this product in 1L distilled water or deionized water
Medium (can increase or decrease proportionally), heat and cook
Boiling until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, cooled to 50 to 55 ℃, add 1 20601 per 100 ml medium (CIN - 1 medium base additives), blender, pour into tablets.
 
Formula: g/L
Tryptone (Tryptone) 20.0g
Sodium Chloride (nacl) 1.0 g
Calcium Extract (Yeast Extract) 2.0g
Mannitol (Mannitol) 20.0g
Sodium Desoxycholate 2.0 g
Magnesium Sulfate, 7 h2o
(magnesium sulfate •7H2O) 0.01g
Irgasan (Irgasan) 0.0004 g
Neutral Red is 0.03g Neutral
Crystal Violet 0.001g
Agar (Agar) 12.0g
PH7.5 + / - 0.1 (25 ℃)