改良克氏双糖铁培养基配制方法及使用
发布时间:
2022-12-27
作者:
日水生物
改良克氏双糖铁培养基配制方法:
称取本品65.0g于1L蒸馏水或去离子水中(可按比例增加或减少配制量),加热煮沸至完全溶解,分装试管,121℃高压灭菌15分钟,灭菌结束后制成高层斜面,备用。
Formula(改良克氏双糖铁培养基配方): g/L
Peptone (蛋白胨) 20.0g
Beef Extract(牛肉浸出粉) 3.0g
Yeast Extract(酵母浸粉) 3.0g
Sodium Chloride(氯化钠) 5.0g
Sorbitol(山梨醇) 20.0g
Dextrose(葡萄糖) 1.0g
Ferric Ammonium Citrate
(柠檬酸铁铵) 0.5g
Sodium Thiosulfate(硫代硫酸钠) 0.5g
Phenol Red(酚红) 0.025g
Agar(琼脂) 12.0g
pH7.4±0.2(25℃)
Preparation method:
Weigh 65.0g of this product in 1L distilled water or deionized water
Medium (can increase or decrease proportionally), heat and cook
Boiling until completely dissolved, repackaging tube, 121 ℃ high pressure sterilization for 15 minutes, after the sterilization cant made top, set aside.
Formula: g/L
Peptone (Peptone) 20.0g
Beef Extract 3.0g
Calcium Extract (Yeast Extract) 3.0g
Sodium Chloride (nacl) 5.0 g
Sorbitol (Sorbitol) 20.0g
Dextrose (glucose) 1.0g
Ferric Ammonium Citrate
(ammonium ferric citrate) 0.5g
Sodium Thiosulfate (Sodium Thiosulfate) was 0.5g
Phenol Red (Phenol Red) 0.025g
Agar (Agar) 12.0g
PH7.4 slightly + / - 0.2 (25 ℃)