高盐察氏培养基配制

发布时间:

2022-12-27

作者:

日水生物


配制方法和配制过程:称取本品110.0g于1L蒸馏水或去离子水中,加热煮沸至完全溶解,分装,121℃高压灭菌20分钟,灭菌结束后请摇匀,以防琼脂沉积于器皿底部而凝固,备用。
Formula(配方): g/L
Sodium Nitrate(硝酸钠)2.0g
Dipotassium phosphate1.0g
(磷酸氢二钾)
Magnesium Sulfate(硫酸镁)0.5g
Potassium Chloride(氯化钾)0.5g
Ferrous Sulfate(硫酸亚铁)0.01g
Sodium Chloride(氯化钠)60.0g
Sucrose(蔗糖) 30.0g
Agar(琼脂) 16.0g          
pH6.0±0.2(25℃)       
 

Preparation method:

According to take this product 110.0 g in 1 l of distilled water or deionized water, heat to boil until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 20 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.
 
Formula: g/L
Sodium Nitrate 2.0g
Dipotassium phosphate 1.0 g
(dipotassium hydrogen phosphate)
Magnesium Sulfate, Magnesium Sulfate) 0.5 g
Potassium Chloride (KCL) 0.5 g
Ferrous Sulfate (Ferrous Sulfate) 0.01g
Sodium Chloride (nacl) 60.0 g
Sucrose (Sucrose) 30.0g
Agar(Agar) 16.0g
PH6.0 + / - 0.2 (25 ℃)