抗生素检定培养基Ⅰ号(高 pH)(pH7.8-8.0)配制方法

发布时间:

2022-12-27

作者:


抗生素检定培养基Ⅰ号(高 pH)(pH7.8-8.0)英文名称:Antibiotic Agar No.1(High pH)
产品编号:11803
产品规格:250g/瓶
产品用途:用于链霉素、卡那霉素、新霉素等的效价测定
 
Formula(抗生素检定培养基Ⅰ号(高 pH)(pH7.8-8.0)配方): g/L
Peptone(蛋白胨)         5.0g
Beef Extract(牛肉浸出粉)  3.0g
Dipotassium Phosphate(磷酸氢二钾)   3.0g
Agar(琼脂)                18.0g
pH7.9±0.1(25℃)  
 
培养基的配制方法:
    称取本品29.0g于1L蒸馏水或去离子水中,加热搅拌煮沸至完全溶解,分装,115℃高压灭菌30分钟,灭菌结束后请摇匀,以防琼脂沉积于器皿底部而凝固,备用。

 


 

(1) policy Agar No.1 (High pH)
Product number: 11803
Product specification: 250g/ bottle
Purpose: to determine the titer of streptomycin, kanamycin, neomycin, etc
 
Formula: g/L
Peptone (Peptone) 5.0g
Beef Extract 3.0g
Dipotassium Phosphate, potassium hydrogen Phosphate) 3.0 g
Agar (Agar) 18.0g
PH7.9 + / - 0.1 (25 ℃)
 
Preparation method:
According to take this product 29.0 g in 1 l of distilled water or deionized water, heating boiling stir until completely dissolved, repackaging, 115 ℃ high pressure sterilization for 30 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.