十六烷基三甲基溴化铵培养基配方

发布时间:

2022-12-27

作者:


十六烷基三甲基溴化铵培养基英文名称:Cetrimide  Medium
产品编号:11705
产品规格:250g/瓶
产品用途:用于绿脓杆菌分离培养
 
Formula(十六烷基三甲基溴化铵培养基配方): g/L
Peptone(蛋白胨 )           10.0g
Beef Extract(牛肉浸粉)        3.0g
Sodium Chloride(氯化钠)       5.0g
Cetrimide(十六烷基三甲基溴化铵)  0.3g
Agar(琼脂)                    20.0g
pH7.5±0.1(25℃)
 
配制方法:
    称取本品38.3g于1L蒸馏水或去离子水中(可按比例增加或减少配制量),加热煮沸至完全溶解,分装,115℃高压灭菌20分钟,灭菌结束后请摇匀,以防琼脂沉积于器皿底部而凝固,备用。

 

 


English name: Cetrimide Medium
Product number: 11705
Product specification: 250g/ bottle
Purpose: for isolation and culture of pseudomonas aeruginosa
 
Formula: g/L
Peptone (Peptone) 10.0g
Beef Extract 3.0g
Sodium Chloride (nacl) 5.0 g
Cetrimide(ammonium hexadecyl trimethyl bromide) 0.3g
Agar(Agar) 20.0g
PH7.5 + / - 0.1 (25 ℃)
 
Preparation method:
According to take this product 38.3 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling until completely dissolved, repackaging, 115 ℃ high pressure sterilization for 20 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.