溴化十六烷基三甲铵琼脂培养基基础配方

发布时间:

2022-12-27

作者:


英文名称:Cetrimide  Agar  Medium
产品编号:11735-1
产品规格:250g/瓶
产品用途:用于铜绿假单胞菌的选择性分离
 
Formula(配方): g/L
Pancreatic Digest of Gelatin(明胶胰酶水解        20.0g
Potassium Sulfate(硫酸钾)        10.0g
Magnesium Chloride(氯化镁)      1.4g
Cetrimide (溴化十六烷基三甲铵)     0.3g
Agar(琼脂)                                  13.6g
pH7.4±0.2(25℃)
 
培养基的配制方法:
    称取本品4.53g于100ml蒸馏水或去离子水中,添加1支21702(甘油),加热煮沸至完全溶解,121℃高压灭菌15分钟,灭菌结束后请摇匀,以防琼脂沉积于器皿底部而凝固。
 
   

  
 
 
 
Cetrimide Agar Medium
Product number: 11735-1
Product specification: 250g/ bottle
Application: used for selective isolation of pseudomonas aeruginosa
 
Formula: g/L
Pancreatic Digest of sunlight (gelatine trypsin hydrolysis 20.0g)
Potassium Sulfate, Potassium Sulfate) 10.0 g
Magnesium Chloride, Magnesium Chloride) 1.4 g
Cetrimide (hexadecyl trimethylammonium bromide) 0.3g
Agar(Agar) 13.6g
PH7.4 slightly + / - 0.2 (25 ℃)
 
 
Preparation method:
Said the 4.53 g amount of this product in 100 ml of distilled water or deionized water, add 1-21702 (glycerin), heat to boil until completely dissolved, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification.