沙氏葡萄糖琼脂培养基SDA氯霉素配方

发布时间:

2022-12-27

作者:

山东培养基公司日水生物


沙氏葡萄糖琼脂培养基SDA氯霉素配制方法:称取本品65.1g于1L蒸馏水或去离子水中,加热煮沸至完全溶解,分装,121℃高压灭菌15分钟,灭菌结束后请摇匀,以防琼脂沉淀于器皿底部而凝固,备用。

Sabouraud’s Glucose Agar Medium with Chloramphenicol Formula[配方]: g/L
Mixture of Peptic Digest of Animal Tissue
and Pancreatic Digest of  Casein [1:1)[动物组织胃蛋白酶水解物和胰酪胨等量混合物]:10.0g
Dextrose[葡萄糖]:40.0g
Chloramphenicol[氯霉素]:0.1g
Agar[琼脂]:15.0g
pH5.6±0.2[25℃]
 
Sachs glucose agar medium SDA chloramphenicol preparation method: 65.1g in 1L distilled water or deionized water, heated and boiled until completely dissolved, sub-packed, high-pressure sterilization at 121 ~C for 15 minutes, after sterilization, shake well, in order to prevent agar precipitation in the bottom of the vessel and solidification, reserve.
Sabouraud 's Glucose Agar Medium with Chloramphenicol Formula[formula]:g/L
Mixture of Peptic Digest of Animal Tissue
Pancreatic Digest of Casein [1:1] [Mixture of pepsin hydrolysate and peptone in animal tissues]: 10.0g
Dextrose[glucose]:40.0g
Chloramphenicol[chloramphenicol]:0.1g
Agar[agar]:15.0g
PH5.6 + 0.2[25 C]